Biomedical & Life Sciences

Every cell, seen and measured.
Every result, traceable and reproducible.

OptiFlow is a domain-aware image-analytics platform for life-science imaging — from light, fluorescence and confocal microscopy to super-resolution and whole-slide pathology. Build the analysis visually, quantify what you see, and run it from a single image to a whole cohort — exporting results that carry the exact method that made them. No code, 100% local.

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In production at ETH Zürich CytoByte label-free staining 20+ peer-reviewed publications Featured by CNA & The Straits Times
The analysis gap

Your images hold the answer.
Getting it out — reliably — is the hard part.

Life-science imaging has outpaced the tools used to analyse it. Methods grow so intricate they no longer fit in a paragraph of a methods section; biologists are pushed into scripting they never signed up for; and the volumes from high-content screens and whole-slide scanners are far beyond what anyone can review by hand.

Methods you can't reproduce

A multi-step analysis rarely survives the journey from one operator, instrument, or paper to the next — and a result you can't reproduce is a result you can't defend.

Experts stuck on plumbing

Researchers lose experimental time to image processing and scripting instead of the biology they were hired for — and pipelines break the moment the person who built them moves on.

Scale beyond manual review

Whole-slide and high-content datasets are too large to inspect exhaustively, yet too important to sample — so the rare, decisive event is the one most easily missed.

Who we serve

One platform.
Built around your work.

Biomedical imaging spans very different teams with very different goals — from a single time-point to longitudinal, live-cell studies — but the same need for analysis they can trust, repeat, and defend. OptiFlow adapts to each, rather than forcing your work into a generic mould.

Academic & translational research

Reproducible analytics, in the researcher's hands — no scripting, no single point of failure.
  • Single-cell & morphometric profiling
  • Live-cell & time-lapse imaging
  • Methods you can publish and re-run

Drug discovery & screening

From high-content screens to confident decisions — at the throughput a campaign demands.
  • Phenotypic profiling at scale
  • Hit-calling & dose–response
  • Plate & batch normalisation

Cell & gene therapy / bioprocessing

Watch your cells — without touching them. Non-invasive readouts throughout the process.
  • Label-free viability & differentiation readouts
  • Batch-to-batch & line-to-line consistency
  • Non-perturbative in-process QC

Diagnostics, pathology & cytology

Precision review at whole-slide scale — consistent, exhaustive, and defensible.
  • WSI segmentation & staging
  • Rare-cell & mitotic-figure detection
  • Consistent criteria across sites

Imaging biomarkers & precision medicine

Turn morphology into a measurable readout — classifiers and regressions trained on your own cohort.
  • Morphological & textural signatures
  • Cellular ageing & senescence readouts
  • Label-free progression & grading

A different problem?

If your imaging challenge isn't here, it's likely still a fit. We build for new domains regularly.
Signature capability

Visualise, measure, characterise.
No stain required.

OptiFlow's CytoByte engine performs virtual nuclear staining directly from label-free DIC or phase contrast — in real time, with no chemistry and no phototoxicity, revealing native biology undisturbed. Because the cells are never perturbed, live and longitudinal experiments stay clean.

Label-free DIC microscopy — cells visible only as phase rings The same field after CytoByte virtual staining — nuclei rendered in high contrast on black Virtual nuclei · CytoByte DIC · label-free
A real CytoByte result — drag to compare. No stain was applied; nuclear structure is computed directly from the label-free DIC image.

  • No phototoxicity

    Image live cells as often as you like, without the photodamage a fluorescent stain introduces.

  • No reagents, no prep

    No dyes, no incubation, no fixation — skip the staining workflow and its cost and variability entirely.

  • Non-sacrificial by default

    Image the sample as it is, take your readout, and return it straight to the incubator — nothing is stained, fixed, or consumed.

  • Built on published research

    The science behind CytoByte is peer-reviewed and proven in the field — not a marketing claim.

  • Live AR view: cyan virtual nuclei overlaid on the raw DIC microscope feed in real time

    Real-time virtual staining

    Stream the virtual staining live as the microscope acquires each frame — overlay the real and virtual channels for an AR view of your structure of interest.

In production at ETH Zürich
The biomedical toolkit

Every step, from raw frame
to the decision.

Compose exactly the pipeline your assay needs from a deep library of domain-aware operations — purpose-built for quantitative microscopy. Preview any of them on your own images, then license only what your work demands.

Modalities covered Light & brightfieldMulti-channel fluorescenceConfocalSuper-resolutionPhase contrastDIC3D / z-stackLive-cell time-lapseWhole-slide (WSI)H&EEM (TEM/SEM/STEM)

Restore & enhance

Recover signal from difficult images.

Denoising that is classical, AI-guided, and hybrid — NLM, bilateral, wavelet, total-variation and BM3D for low-light live-cell and EM data. Illumination and contrast correction, CLAHE, and frequency-domain filtering for clean, comparable inputs.

Expanded

Segment & measure

Find every cell and structure.

Otsu, multi-Otsu and adaptive thresholding; morphology and connected-component labelling; ridge filters (Frangi, Sato, Meijering) for vessels, neurites and filaments. Calibrated area, distance and interactive line-intensity profiles — in real units, read from your metadata.

New

Track & quantify over time

Quantify dynamics, not just snapshots.

Track objects across frames and through z — motility, migration, division and lineage — turning live-cell time-lapse and 3D series into quantitative kinetic and spatiotemporal readouts.

New

Classify & grade

Decisions trained on your data.

Supervised deep learning for cell type, phenotype, or disease grade — trained on your own annotated images, so the model learns your problem rather than approximating it.

Rare-event & anomaly detection

Find the needle in the slide.

Exhaustive, slide-wide search for low-prevalence targets — circulating tumour cells, mitotic figures — plus unsupervised anomaly detection that flags the unexpected with no labels required.

Quantify, profile & report

From one cell to a whole cohort.

Per-cell and population morphometrics, enumeration and texture analysis with batch normalisation built in — then built-in plotting (histogram, scatter, box-plot, violin, CDF) and automatic export to Excel and formatted PDF reports.

New

Plus the building blocks every pipeline leans on

Channel and colour operations, lookup tables, geometry and alignment, type conversion, and pixel-wise image maths and compositing — the connective tissue that lets any workflow fit together exactly as your assay requires.

Modular by design — start with what today's work needs and add modules as your assays grow, instrument by instrument and lab by lab. Every operation runs locally, on your own machines. See the full OptiFlow platform.

Reproducible & auditable

Results you can defend.
Methods you can hand over.

Modern image analysis has become too complex to write into a methods section. OptiFlow records it for you — so every result is reproducible, every method is portable and submittable as-is, and your data never leaves your control.

Embedded, tamper-evident provenance

Every result carries its full recipe.

The complete pipeline — every operation, parameter, calibration and operator — is written into the image itself, encrypted and readable only inside OptiFlow. An encrypted, rollback-resistant timestamp guards the record; where it matters, lock it to a password only your team holds.

An exportable method, on demand

Submit it, share it, open-source it.

Export the complete pipeline as a standalone file — to attach to a publication, hand to a collaborator, or release alongside open data. Anyone you give it to can reproduce the exact analysis, down to the parameter.

Air-gapped & 100% local

No image ever leaves the machine.

Processing reads and writes your own filesystem directly. No image data crosses the network — no cloud account, no exceptions — so patient data and unpublished results stay exactly where they belong.

See it: the exact method, recovered from any result.

Every result already contains the workflow that made it. So revisiting your own analysis, handing it to a collaborator, satisfying a reviewer, or re-running across a cohort needs nothing rebuilt and nothing re-documented — and the record stays encrypted, readable only inside OptiFlow, and accessible only when you choose.

Preparing live demo…
From research to production

Built on science.
Engineered for production.

Logic Byte's biomedical capabilities trace directly to peer-reviewed research and deployed systems — the foundation beneath every module on this page.

APL Bioengineering · 2021
Label-free single-cell cancer classification
Machine learning identified cancer cells from label-free imaging of intracellular pH — no fluorescent markers — at 95% accuracy in under 35 minutes. Covered by CNA and The Straits Times.
Vision generative AI · ETH Zürich
In-silico staining from DIC microscopy
The science behind CytoByte: virtual nuclear staining computed directly from DIC, extending to phase-contrast and transmitted-light inputs. Deployed commercially; manuscript in preparation.
AI diagnostics · NUS
Image-based biosensor for disease classification
A deep network trained on morphological and textural cell features to classify disease state on microfluidic platforms — the groundwork for image-based biomarker development.
Explore the full research record
How we work

Start on your own images.
Scale when it earns it.

No rip-and-replace, no leap of faith. Prove the value on your own data first, then grow module by module, lab by lab.

Explore Deploy Expand Embed
Preview before you buy 60-day module guarantee Priority support, included Pay only for what you use

See it on your images.

Bring a biomedical imaging problem and your own data — we'll show you exactly what OptiFlow does with them. If your workflow needs something we haven't built yet, we'll build it.

Book a Demo Talk to us first
In production at ETH Zürich Ongoing trials at NTU 20+ peer-reviewed publications Featured by CNA & The Straits Times